Description

3x Flag represents an enhanced version of the Flag-Tag system, consisting of three tandem copies of the DYKDDDDK sequence separated by appropriate linker regions to provide amplified epitope recognition and improved purification efficiency. The triple Flag design significantly increases the binding capacity for anti-Flag antibodies, resulting in enhanced sensitivity for protein detection and more efficient immunoaffinity purification compared to single Flag tags. The peptide maintains the hydrophilic characteristics of the original Flag sequence while providing multiple binding sites for antibody recognition. The 3x Flag system is particularly valuable for applications requiring high-sensitivity detection or purification of low-abundance proteins. Research applications include enhanced protein purification protocols, sensitive protein detection assays, and studies requiring high-efficiency protein isolation from complex biological samples. For research use only.